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Macromolecule Content Total Structure Weight: This is version 1. Structural and thermodynamic characterization of pre- and postpolymerization states in the F4 fimbrial subunit FaeG Van Molle, I.
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No landline or engineer visit. SIM only. Mobile Phones. Mobile Broadband. Home Broadband. Using in vitro assays in combination with antibodies and molecular markers that define cellular components of the developing spinal cord, we have identified several components of a guidance system that may contribute to the projection pattern of a subset of sensory relay neurons, the commissural neurons.
Commissural neurons project circumferentially and ventrally away from the dorsal midline of the neural tube and towards and across the ventral midline of the spinal cord before turning to project towards higher spinal segments and supraspinal sites.
Two groups of cells, located at the dorsal and ventral midlines and known as the roof plate and the floor plate, respectively, appear to act as sources of cues for commissural axons.
The roof plate appears to direct initial axon extension away from the dorsal midline through the release of a diffusible chemorepellent, whereas the floor plate provides both contact-mediated and diffusible cues for commissural axon navigation towards and across the midline.
We are currently trying to determine how roof plate- and floor plate-derived signals act to influence the direction of growth of commissural axons.
The ventral midline of the neural tube consists of several subgroups of cells that differ, according to their position in the rostro-caudal axis, in the expression of cellular markers and signaling molecules.
Throughout the axis, these cells appear to have multiple roles in the patterning of adjacent cell types and axons. To begin to study the mechanisms underlying cell identity within the neural tube we have therefore examined the development of the ventral midline cell groups.
By analyzing changes in neural cell identity and pattern within neural tube explants, we have shown that axial mesodermal cells of the notochord and prechordal mesoderm provide inductive signals that initiate the differentiation of the ventral midline and patterning of neurons within the neural plate.